I'm fucking DONE with syringe filters and want to switch to bottle top! What supplies do I need, from where and what is the process? Thanks.
Is this pretty much everything I need?
Nalgene Nylon Bottle Filter 0.2µm, 150 mL, Sterile
Nalgene Nylon Bottle Filter, 0.2um, 150 mL, Sterilebuyneedles.ca
google it and you will find some videos on it. That is what I always do when presented with a "How to" question
yes that filter is fine and so is the vacuum pump. if your doing any sort of volume you will want an electric pump. try amazon for that
@Furius_Saturninus_Plautia are u using whatman? .45 or .20um?Not much volume, I'm just tired of syringe filters breaking and fucking everything up so on to bottle top system
totally agree with the electric pump. if it is not to ,much of a cost.google it and you will find some videos on it. That is what I always do when presented with a "How to" question
yes that filter is fine and so is the vacuum pump. if your doing any sort of volume you will want an electric pump. try amazon for that
@Furius_Saturninus_Plautia are u using whatman? .45 or .20um?
I always used .45 micron. Never had any bad batches. I tried the smaller micron I think it might have been .22 and damn could not get the gear through the filter no matter how hot I kept the mixture. Never had any pip issues using the .45 and I would get about 5 10 mls btls out of a .45 filter. Anyway just a thought. I was going to switch to a pump ... but if you are doing small batches like 10 10ml at a time the .45 is adequate. The first 3 bottles are really quick until the filter starts filling up with shit on the 4th and 5th..22 whatman.
I always used .45 micron. Never had any bad batches. I tried the smaller micron I think it might have been .22 and damn could not get the gear through the filter no matter how hot I kept the mixture. Never had any pip issues using the .45 and I would get about 5 10 mls btls out of a .45 filter. Anyway just a thought. I was going to switch to a pump ... but if you are doing small batches like 10 10ml at a time the .45 is adequate. The first 3 bottles are really quick until the filter starts filling up with shit on the 4th and 5th.
worked for me and I had no issues. What else are you catching with a .22... a sore hand and blow outs. Was not worth it for me.
What electric vacuum pump are you guys suggesting ?
Seen this on amazon
Looks cheap as fuck https://www.amazon.com/gp/aw/d/B083...ZCTQ6SW#aw-udpv3-customer-reviews_feature_div
OR
JOANLAB Oilless Diaphragm Vacuum Pump Portable Negative Pressure Vacuum Pump Industrial Lab Vacuum Filtration Pump10L/min,with Rubber Tube: Amazon.com: Industrial & Scientific
JOANLAB Oilless Diaphragm Vacuum Pump Portable Negative Pressure Vacuum Pump Industrial Lab Vacuum Filtration Pump10L/min,with Rubber Tube: Amazon.com: Industrial & Scientificwww.amazon.com
1hour ?! Thats a cheap alternative tho.. Brake bleeders are like 20-40$Brake bleeders. Two at a time to save time as it takes over an hour to do 500ml. You will need to get up to about 25psi for 22 micron filters
Oh shit ok.. Is 11psi enough to filter or not even..Be careful you don't use too much negative pressure. The viscosity will determine pass rate through the filter. I use GSO but if you use say EO then the rate of which is passes through the filter is much quicker. Warmer is good but not too hot. Be careful you don't use nylon filters with glycol. They will melt. Is the electric vacuum adjustable?
Edit- I see that Joanlab is 0.075Mpa so it's just under 11psi. That's a bit less than half of what you may need.
Oh shit ok.. Is 11psi enough to filter or not even..